FSHR polymorphism p.N680S mediates different responses to FSH in vitro.
Mol Cell Endocrinol
; 393(1-2): 83-91, 2014 Aug 05.
Article
em En
| MEDLINE
| ID: mdl-24970684
ABSTRACT
The single nucleotide polymorphism p.N680S of the follicle-stimulating hormone (FSH) receptor (FSHR) is a discrete marker of ovarian response but previous in vitro studies failed to demonstrate differences in the response to FSH between N and S carrier cells. Here we demonstrate that p.N680S mediates different kinetics of the response to FSH in vitro. Intracellular cAMP production is faster in p.N680S N than in S homozygous human granulosa cells (45 versus 90 min to achieve the plateau, respectively; Mann-Whitney's U-test; p < 0.005; n = 4). Reflecting the cAMP kinetics, phospho-ERK1/2 and -CREB activation, AREG and STARD1 gene expressions and progesterone production were qualitatively and quantitatively different in N versus S homozygous cells. Finally, the blockade of ERK pathway by U0126 abolishes the genotype-mediated different effects on gene expression and progesterone production (Mann-Whitney's U-test; p ≥ 0.005; n = 3).
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Polimorfismo Genético
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Receptores do FSH
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Transdução de Sinais
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Regulação da Expressão Gênica
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Hormônio Foliculoestimulante
Limite:
Animals
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Female
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Humans
Idioma:
En
Revista:
Mol Cell Endocrinol
Ano de publicação:
2014
Tipo de documento:
Article