Differentiation-dependent requirement of Tsix long non-coding RNA in imprinted X-chromosome inactivation.
Nat Commun
; 5: 4209, 2014 Jun 30.
Article
em En
| MEDLINE
| ID: mdl-24979243
ABSTRACT
Imprinted X-inactivation is a paradigm of mammalian transgenerational epigenetic regulation resulting in silencing of genes on the paternally inherited X-chromosome. The preprogrammed fate of the X-chromosomes is thought to be controlled in cis by the parent-of-origin-specific expression of two opposing long non-coding RNAs, Tsix and Xist, in mice. Exclusive expression of Tsix from the maternal-X has implicated it as the instrument through which the maternal germline prevents inactivation of the maternal-X in the offspring. Here, we show that Tsix is dispensable for inhibiting Xist and X-inactivation in the early embryo and in cultured stem cells of extra-embryonic lineages. Tsix is instead required to prevent Xist expression as trophectodermal progenitor cells differentiate. Despite induction of wild-type Xist RNA and accumulation of histone H3-K27me3, many Tsix-mutant X-chromosomes fail to undergo ectopic X-inactivation. We propose a novel model of lncRNA function in imprinted X-inactivation that may also apply to other genomically imprinted loci.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cromossomo X
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Diferenciação Celular
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Impressão Genômica
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Inativação do Cromossomo X
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RNA Longo não Codificante
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Camundongos
Limite:
Animals
Idioma:
En
Revista:
Nat Commun
Ano de publicação:
2014
Tipo de documento:
Article