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Structural analysis and characterization of new small serum proteins from the serum of a venomous snake (Gloydius blomhoffii).
Shioi, Narumi; Deshimaru, Masanobu; Terada, Shigeyuki.
Afiliação
  • Shioi N; a Faculty of Science, Department of Chemistry , Fukuoka University , Jonan-ku, Fukuoka , Japan.
Biosci Biotechnol Biochem ; 78(3): 410-9, 2014.
Article em En | MEDLINE | ID: mdl-25036827
ABSTRACT
Some snakes have several anti-toxic proteins in their sera that neutralize their own venom. Five new small serum proteins (SSPs) were isolated from Japanese mamushi (Gloydius blomhoffii) serum by gel-filtration and RP-HPLC, and their N-Terminal sequences were determined. The amino acid sequences of the precursor proteins were deduced from the nucleotide sequences of cDNAs encoding them. Due to the sequence similarity to those of SSPs in habu snake (Protobothrops flavoviridis) serum (>75% identity), these proteins were designated mSSP-1 to mSSP-5 as the homologs of habu proteins. mSSP-1 was stable at 100 °C and in the pH range of 1-10, and inhibited the proteolytic activity of a certain snake venom metalloproteinase. The inhibitory activity was extinguished by modifying the amino groups of mSSP-1. mSSP-1 is the first prostate secretory protein of the 94 amino acid-family protein with a carbohydrate chain in the Asn37 residue.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Venenos de Serpentes / Proteínas Sanguíneas Limite: Animals Idioma: En Revista: Biosci Biotechnol Biochem Ano de publicação: 2014 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Venenos de Serpentes / Proteínas Sanguíneas Limite: Animals Idioma: En Revista: Biosci Biotechnol Biochem Ano de publicação: 2014 Tipo de documento: Article