Global and gene-specific DNA methylation pattern discriminates cholecystitis from gallbladder cancer patients in Chile.
Future Oncol
; 11(2): 233-49, 2015.
Article
em En
| MEDLINE
| ID: mdl-25066711
ABSTRACT
AIM:
The aim of the study was to evaluate the use of global and gene-specific DNA methylation changes as potential biomarkers for gallbladder cancer (GBC) in a cohort from Chile. MATERIAL &METHODS:
DNA methylation was analyzed through an ELISA-based technique and quantitative methylation-specific PCR.RESULTS:
Global DNA Methylation Index (p = 0.02) and promoter methylation of SSBP2 (p = 0.01) and ESR1 (p = 0.05) were significantly different in GBC when compared with cholecystitis. Receiver curve operator analysis revealed promoter methylation of APC, CDKN2A, ESR1, PGP9.5 and SSBP2, together with the Global DNA Methylation Index, had 71% sensitivity, 95% specificity, a 0.97 area under the curve and a positive predictive value of 90%.CONCLUSION:
Global and gene-specific DNA methylation may be useful biomarkers for GBC clinical assessment.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Colecistite
/
Metilação de DNA
/
Neoplasias da Vesícula Biliar
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Adult
/
Aged
/
Aged80
/
Female
/
Humans
/
Male
/
Middle aged
País/Região como assunto:
America do sul
/
Chile
Idioma:
En
Revista:
Future Oncol
Ano de publicação:
2015
Tipo de documento:
Article