Three-dimensional arbitrary trajectory scanning photoacoustic microscopy.
J Biophotonics
; 8(4): 303-8, 2015 Apr.
Article
em En
| MEDLINE
| ID: mdl-25077689
ABSTRACT
We have enhanced photoacoustic microscopy with three-dimensional arbitrary trajectory (3-DAT) scanning, which can rapidly image selected vessels over a large field of view (FOV) and maintain a high signal-to-noise ratio (SNR) despite the depth variation of the vessels. We showed that hemoglobin oxygen saturation (sO2 ) and blood flow can be measured simultaneously in a mouse ear in vivo at a frame rate 67 times greater than that of a traditional two-dimensional raster scan. We also observed sO2 dynamics in response to switching from systemic hypoxia to hyperoxia. 3-DAT-scanning photoacoustic microscopy. Schematic diagram of the 3D scanning stage and method.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Imageamento Tridimensional
/
Técnicas Fotoacústicas
/
Microscopia
Limite:
Animals
/
Humans
Idioma:
En
Revista:
J Biophotonics
Ano de publicação:
2015
Tipo de documento:
Article