Accumulation of the insecticidal crystal protein of Bacillus thuringiensis subsp. kurstaki in post-exponential-phase Bacillus subtilis.
Appl Environ Microbiol
; 55(9): 2302-7, 1989 Sep.
Article
em En
| MEDLINE
| ID: mdl-2508556
ABSTRACT
A gene from Bacillus thuringiensis subsp. kurstaki that codes for a Lepidoptera-specific insecticidal toxin (delta-endotoxin) was engineered for expression in Bacillus subtilis. A low-copy-number plasmid vector that replicates in Escherichia coli and B. subtilis was constructed to transform B. subtilis with gene fusions first isolated and characterized in E. coli. Naturally occurring promoter sequences from B. subtilis (43, veg, ctc, and spoVG) were inserted upstream from the plasmid-borne structural gene. In the most prolific case, when the sporulation-specific spoVG promoter was fused to the heterologous toxin gene, the toxin product accumulated during postexponential growth to greater than 25% of the total cell protein. However, the resulting specific activity of the insecticidal toxin product was not commensurate with the abundance of the protein.
Texto completo:
1
Coleções:
01-internacional
Contexto em Saúde:
2_ODS3
Base de dados:
MEDLINE
Assunto principal:
Bacillus subtilis
/
Bacillus thuringiensis
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Proteínas de Bactérias
/
Toxinas Bacterianas
/
Endotoxinas
/
Lepidópteros
Limite:
Animals
Idioma:
En
Revista:
Appl Environ Microbiol
Ano de publicação:
1989
Tipo de documento:
Article