Next-generation sequencing of multiple individuals per barcoded library by deconvolution of sequenced amplicons using endonuclease fragment analysis.

Andersen, Jeppe D; Pereira, Vania; Pietroni, Carlotta; Mikkelsen, Martin; Johansen, Peter; Børsting, Claus; Morling, Niels

Andersen, Jeppe D; Pereira, Vania; Pietroni, Carlotta; Mikkelsen, Martin; Johansen, Peter; Børsting, Claus; Morling, Niels.

Afiliação

Andersen JD; Section of Forensic Genetics, Department of Forensic Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
Pereira V; Section of Forensic Genetics, Department of Forensic Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
Pietroni C; Section of Forensic Genetics, Department of Forensic Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
Mikkelsen M; Section of Forensic Genetics, Department of Forensic Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
Johansen P; Section of Forensic Genetics, Department of Forensic Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
Børsting C; Section of Forensic Genetics, Department of Forensic Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
Morling N; Section of Forensic Genetics, Department of Forensic Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.

Biotechniques ; 57(2): 91-4, 2014 Aug.

Article em En | MEDLINE | ID: mdl-25109295

Assuntos

Endonucleases/genética; Sequenciamento de Nucleotídeos em Larga Escala/métodos; Receptor Tipo 1 de Melanocortina/genética; DNA/genética; Código de Barras de DNA Taxonômico; Técnicas de Genotipagem/métodos; Humanos

Palavras-chave

DNA library preparation; amplicons; melanocortin 1 receptor (MC1R); next-generation sequencing; restriction endonucleases

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Receptor Tipo 1 de Melanocortina / Endonucleases / Sequenciamento de Nucleotídeos em Larga Escala Limite: Humans Idioma: En Revista: Biotechniques Ano de publicação: 2014 Tipo de documento: Article

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