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Ribosylhopane, a novel bacterial hopanoid, as precursor of C35 bacteriohopanepolyols in Streptomyces coelicolor A3(2).
Liu, Wenjun; Sakr, Elias; Schaeffer, Philippe; Talbot, Helen M; Donisi, Janina; Härtner, Thomas; Kannenberg, Elmar; Takano, Eriko; Rohmer, Michel.
Afiliação
  • Liu W; Université de Strasbourg/CNRS, Institut Le Bel, 4 rue Blaise Pascal, 67070 Strasbourg, Cedex (France).
Chembiochem ; 15(14): 2156-61, 2014 Sep 22.
Article em En | MEDLINE | ID: mdl-25155017
Wild-type Streptomyces coelicolor A3(2) produces aminobacteriohopanetriol as the only elongated C35 hopanoid. The hopanoid phenotype of two mutants bearing a deletion of genes from a previously identified hopanoid biosynthesis gene cluster provides clues to the formation of C35 bacteriohopanepolyols. orf14 encodes a putative nucleosidase; its deletion induces the accumulation of adenosylhopane as it cannot be converted into ribosylhopane. orf18 encodes a putative transaminase; its deletion results in the accumulation of adenosylhopane, ribosylhopane, and bacteriohopanetetrol. Ribosylhopane was postulated twenty years ago as a precursor for bacterial hopanoids but was never identified in a bacterium. Absence of the transaminase encoded by orf18 prevents the reductive amination of ribosylhopane into aminobacteriohopanetriol and induces its accumulation. Its reduction by an aldose-reductase-like enzyme produces bacteriohopanetetrol, which is normally not present in S. coelicolor.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Triterpenos / Streptomyces coelicolor Idioma: En Revista: Chembiochem Ano de publicação: 2014 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Triterpenos / Streptomyces coelicolor Idioma: En Revista: Chembiochem Ano de publicação: 2014 Tipo de documento: Article