Modulation of cisplatin sensitivity in human ovarian carcinoma A2780 and SKOV3 cell lines by sulforaphane.
Toxicol Lett
; 230(3): 479-86, 2014 Nov 04.
Article
em En
| MEDLINE
| ID: mdl-25159039
ABSTRACT
Cisplatin resistance is one of the major obstacles in the treatment of ovarian cancer. In an effort to look for new possibilities of how to overcome this difficulty, we studied the mechanisms of the interactions between sulforaphane (SFN) and cisplatin (cisPt) in combined treatment of human ovarian carcinoma A2780 and SKOV3 cell lines. Synergy (A2780) and antagonism (SKOV3) found in MTT assay was confirmed by apoptosis. While SFN significantly potentiated cisPt-induced DNA damage in A2780 cells, it protected SKOV3 cells against cisPt-crosslinking. We revealed a less efficient Nrf-2 pathway inducibility by SFN in A2780 compared to SKOV3 cells, when activation of the Nrf-2 pathway incites its protectivity against cisPt. Thus, different activation of the Nrf-2 pathway may explain the dual effects of SFN.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Neoplasias Ovarianas
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Cisplatino
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Isotiocianatos
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Resistencia a Medicamentos Antineoplásicos
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Antineoplásicos
Tipo de estudo:
Diagnostic_studies
Limite:
Female
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Humans
Idioma:
En
Revista:
Toxicol Lett
Ano de publicação:
2014
Tipo de documento:
Article