Your browser doesn't support javascript.
loading
Levels and complexity of IgA antibody against oral bacteria in samples of human colostrum.
Petrechen, L N; Zago, F H; Sesso, M L T; Bertoldo, B B; Silva, C B; Azevedo, K P; de Lima Pereira, S A; Geraldo-Martins, V R; Ferriani, V P L; Nogueira, R D.
Afiliação
  • Petrechen LN; Department of Pediatrics, Medical School of Ribeirao Preto, University of Sao Paulo, Sao Paulo, Brazil. Electronic address: liara@portalnosralla.com.br.
  • Zago FH; Uberaba University, Uberaba, Minas Gerais, Brazil.
  • Sesso ML; Department of Pediatrics, Medical School of Ribeirao Preto, University of Sao Paulo, Sao Paulo, Brazil. Electronic address: malutase@yahoo.com.br.
  • Bertoldo BB; Uberaba University, Uberaba, Minas Gerais, Brazil. Electronic address: barbarabellochiob@hotmail.com.
  • Silva CB; Uberaba University, Uberaba, Minas Gerais, Brazil. Electronic address: beatriz.camilla@hotmail.com.
  • Azevedo KP; Uberaba University, Uberaba, Minas Gerais, Brazil. Electronic address: ka_passaglia@hotmail.com.
  • de Lima Pereira SA; Uberaba University, Uberaba, Minas Gerais, Brazil. Electronic address: sanivia.pereira@uniube.br.
  • Geraldo-Martins VR; Uberaba University, Uberaba, Minas Gerais, Brazil. Electronic address: vinicius.martins@uniube.br.
  • Ferriani VP; Department of Pediatrics, Medical School of Ribeirao Preto, University of Sao Paulo, Sao Paulo, Brazil. Electronic address: vplferri@fmrp.usp.br.
  • Nogueira RD; Department of Pediatrics, Medical School of Ribeirao Preto, University of Sao Paulo, Sao Paulo, Brazil; Uberaba University, Uberaba, Minas Gerais, Brazil. Electronic address: ruchele_nogueira@yahoo.com.br.
Immunobiology ; 220(1): 142-6, 2015 Jan.
Article em En | MEDLINE | ID: mdl-25175558
Streptococcus mutans (SM) have three main virulence antigens: glucan binding protein B (gbpB), glucosyltransferase (Gtf) and antigens I/II (Ag I/II) envolved in the capacity of those bacteria to adhere and accumulate in the dental biofilm. Also, the glycosyltransferases 153 kDa of Streptococcus gordonii (SGO) and 170kDa of Streptococcus sanguinis (SSA) were important antigens associated with the accumulation of those bacterias. Streptococcus mitis (SMI) present IgA1 protease of 202 kDa. We investigated the specificity and levels IgA against those antigens of virulence in samples of human colostrum. This study involved 77 samples of colostrum that were analyzed for levels of immunoglobulian A, M and G by Elisa. The specificity of IgA against extracts of SM and initials colonizators (SSA, SMI, SGO) were analyzed by the Western blot. The mean concentration of IgA was 2850.2 (±2567.2) mg/100 mL followed by IgM and IgG (respectively 321.8±90.3 and 88.3±51.5), statistically different (p<0.05). Results showed that the majority of samples had detectable levels of IgA antibodies to extracts of bacteria antigens and theirs virulence antigens. To SM, the GbpB was significantly lower detected than others antigens of SM (p<0.05). High complexities of response to Ags were identified in the samples. There were no significant differences in the mean number of IgA-reactive Ags between the antigens (p>0.4). So, the breast milk from first hours after birth presented significant levels of IgA specific against important virulence of antigens those oral streptococci, which can disrupt the installation and accumulation process of these microorganisms in the oral cavity.
Assuntos
Palavras-chave

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bactérias / Imunoglobulina A Secretora / Colostro Tipo de estudo: Prognostic_studies Limite: Adult / Female / Humans Idioma: En Revista: Immunobiology Ano de publicação: 2015 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bactérias / Imunoglobulina A Secretora / Colostro Tipo de estudo: Prognostic_studies Limite: Adult / Female / Humans Idioma: En Revista: Immunobiology Ano de publicação: 2015 Tipo de documento: Article