Whole-genome analysis of multienvironment or multitrait QTL in MAGIC.
G3 (Bethesda)
; 4(9): 1569-84, 2014 Sep 18.
Article
em En
| MEDLINE
| ID: mdl-25237109
ABSTRACT
Multiparent Advanced Generation Inter-Cross (MAGIC) populations are now being utilized to more accurately identify the underlying genetic basis of quantitative traits through quantitative trait loci (QTL) analyses and subsequent gene discovery. The expanded genetic diversity present in such populations and the amplified number of recombination events mean that QTL can be identified at a higher resolution. Most QTL analyses are conducted separately for each trait within a single environment. Separate analysis does not take advantage of the underlying correlation structure found in multienvironment or multitrait data. By using this information in a joint analysis-be it multienvironment or multitrait - it is possible to gain a greater understanding of genotype- or QTL-by-environment interactions or of pleiotropic effects across traits. Furthermore, this can result in improvements in accuracy for a range of traits or in a specific target environment and can influence selection decisions. Data derived from MAGIC populations allow for founder probabilities of all founder alleles to be calculated for each individual within the population. This presents an additional layer of complexity and information that can be utilized to identify QTL. A whole-genome approach is proposed for multienvironment and multitrait QTL analysis in MAGIC. The whole-genome approach simultaneously incorporates all founder probabilities at each marker for all individuals in the analysis, rather than using a genome scan. A dimension reduction technique is implemented, which allows for high-dimensional genetic data. For each QTL identified, sizes of effects for each founder allele, the percentage of genetic variance explained, and a score to reflect the strength of the QTL are found. The approach was demonstrated to perform well in a small simulation study and for two experiments, using a wheat MAGIC population.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Triticum
/
Genoma de Planta
/
Locos de Características Quantitativas
/
Modelos Genéticos
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
G3 (Bethesda)
Ano de publicação:
2014
Tipo de documento:
Article