A droplet-based fluorescence polarization immunoassay (dFPIA) platform for rapid and quantitative analysis of biomarkers.
Biosens Bioelectron
; 67: 497-502, 2015 May 15.
Article
em En
| MEDLINE
| ID: mdl-25241152
ABSTRACT
Herein, we describe for the first time the integration of pneumatic micro-pumps with droplet-based microfluidic systems as basic platform for the rapid detection and quantitation of biomarkers. Specifically, we combine this microfluidic platform with fluorescence polarization detection to identify and quantify the potent blood vessel inducing protein bovine angiogenin within cow's milk in high-throughput. The droplet-based fluorescence polarization immunoassay is successful in accurately determining the concentration (4.84±1.21 µg/mL) of bovine angiogenin in cow's milk, affords a 10 fold reduction in dead volumes when compared to conventional droplet-based microfluidic experiments and requires an total sample volume of less than 1 nL.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ribonuclease Pancreático
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Biomarcadores
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Técnicas Biossensoriais
Limite:
Animals
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Humans
Idioma:
En
Revista:
Biosens Bioelectron
Ano de publicação:
2015
Tipo de documento:
Article