Engineering an enhanced, thermostable, monomeric bacterial luciferase gene as a reporter in plant protoplasts.

Cui, Boyu; Zhang, Lifeng; Song, Yunhong; Wei, Jinsong; Li, Changfu; Wang, Tietao; Wang, Yao; Zhao, Tianyong; Shen, Xihui

Cui, Boyu; Zhang, Lifeng; Song, Yunhong; Wei, Jinsong; Li, Changfu; Wang, Tietao; Wang, Yao; Zhao, Tianyong; Shen, Xihui.

Afiliação

Cui B; State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, China; Department of Microbiology, College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, China.
Zhang L; State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, China; Department of Biochemistry and Molecular Biology, College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, China.
Song Y; State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, China; Department of Microbiology, College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, China.
Wei J; State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, China; Department of Biochemistry and Molecular Biology, College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, China.
Li C; State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, China; Department of Biochemistry and Molecular Biology, College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, China.
Wang T; State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, China; Department of Microbiology, College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, China.
Wang Y; State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, China; Department of Biochemistry and Molecular Biology, College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, China.
Zhao T; State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, China; Department of Biochemistry and Molecular Biology, College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, China.
Shen X; State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, China; Department of Microbiology, College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, China.

PLoS One ; 9(10): e107885, 2014.

Article em En | MEDLINE | ID: mdl-25271765

Assuntos

Expressão Gênica; Genes Reporter; Luciferases Bacterianas/genética; Plantas/metabolismo; Protoplastos/metabolismo; Códon; Embaralhamento de DNA; Estabilidade Enzimática; Ordem dos Genes; Genes de Plantas; Concentração de Íons de Hidrogênio; Luciferases Bacterianas/química; Modelos Moleculares; Dados de Sequência Molecular; Mutação; Plantas/genética; Plasmídeos/genética; Conformação Proteica; Proteínas Recombinantes de Fusão/química; Proteínas Recombinantes de Fusão/genética; Proteínas Recombinantes de Fusão/isolamento & purificação; Proteínas Recombinantes de Fusão/metabolismo; Temperatura; Zea mays/genética; Zea mays/metabolismo

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Plantas / Protoplastos / Expressão Gênica / Genes Reporter / Luciferases Bacterianas Idioma: En Revista: PLoS One Ano de publicação: 2014 Tipo de documento: Article

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