Targeted combinatorial alternative splicing generates brain region-specific repertoires of neurexins.
Neuron
; 84(2): 386-98, 2014 Oct 22.
Article
em En
| MEDLINE
| ID: mdl-25284007
ABSTRACT
Molecular diversity of surface receptors has been hypothesized to provide a mechanism for selective synaptic connectivity. Neurexins are highly diversified receptors that drive the morphological and functional differentiation of synapses. Using a single cDNA sequencing approach, we detected 1,364 unique neurexin-α and 37 neurexin-ß mRNAs produced by alternative splicing of neurexin pre-mRNAs. This molecular diversity results from near-exhaustive combinatorial use of alternative splice insertions in Nrxn1α and Nrxn2α. By contrast, Nrxn3α exhibits several highly stereotyped exon selections that incorporate novel elements for posttranscriptional regulation of a subset of transcripts. Complexity of Nrxn1α repertoires correlates with the cellular complexity of neuronal tissues, and a specific subset of isoforms is enriched in a purified cell type. Our analysis defines the molecular diversity of a critical synaptic receptor and provides evidence that neurexin diversity is linked to cellular diversity in the nervous system.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Encéfalo
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RNA Mensageiro
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Éxons
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Processamento Alternativo
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Proteínas do Tecido Nervoso
Limite:
Animals
Idioma:
En
Revista:
Neuron
Ano de publicação:
2014
Tipo de documento:
Article