Unravelling the mechanism of non-ribosomal peptide synthesis by cyclodipeptide synthases.
Nat Commun
; 5: 5141, 2014 Oct 06.
Article
em En
| MEDLINE
| ID: mdl-25284085
ABSTRACT
Cyclodipeptide synthases form cyclodipeptides from two aminoacyl transfer RNAs. They use a ping-pong mechanism that begins with transfer of the aminoacyl moiety of the first aminoacyl tRNA onto a conserved serine, yielding an aminoacyl enzyme. Combining X-ray crystallography, site-directed mutagenesis and affinity labelling of the cyclodipeptide synthase AlbC, we demonstrate that the covalent intermediate reacts with the aminoacyl moiety of the second aminoacyl tRNA, forming a dipeptidyl enzyme, and identify the aminoacyl-binding sites of the aminoacyl tRNAs.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Biossíntese Peptídica
/
Peptídeo Sintases
/
Ribossomos
/
Proteínas de Bactérias
Idioma:
En
Revista:
Nat Commun
Ano de publicação:
2014
Tipo de documento:
Article