Analysis of gene expression and preliminary study of methylation about 11ß-HSD2 gene in placentas of Chinese pre-eclampsia patients of Han ethnicity.
J Obstet Gynaecol Res
; 41(3): 343-9, 2015 Mar.
Article
em En
| MEDLINE
| ID: mdl-25331012
ABSTRACT
AIMS:
The aim of this study was to determine the promoter methylation status of type 2 isoform of 11ß-hydroxysteroid dehydrogenase (11ß-HSD2) and its regulatory correlation with 11ß-HSD2 gene expression in placentas of pre-eclampsia (PE) patients of Chinese Han ethnicity. MATERIAL ANDMETHODS:
The pathological features of placental tissues were studied using hematoxylin-eosin staining and immunohistochemical staining. The 11ß-HSD2 mRNA and protein expressions were detected by real-time polymerase chain reaction and Western blotting. The methylation of the 11ß-HSD2 promoter sequence was examined by bisulfite sequencing polymerase chain reaction.RESULTS:
Trophoblast hyperplasia and discontinuous syncytial layer were observed in the PE group, and the 11ß-HSD2 was distributed irregularly and its immunoreactivity was weakened distinctly. The expressions of 11ß-HSD2 mRNA and protein decreased significantly in the PE group compared with the control group. Unexpectedly, almost no 11ß-HSD2 methylation was detected in PE placental tissue (two fragments, 0.6% vs 0%) or normal placental tissue (1% vs 0.6%). No significant difference in 11ß-HSD2 promoter methylation was found between the two groups.CONCLUSIONS:
The 11ß-HSD2 expression decreased in PE women of Chinese Han ethnicity, but was not interrelated with the promoter methylation status.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Placenta
/
Pré-Eclâmpsia
/
Expressão Gênica
/
Metilação de DNA
/
11-beta-Hidroxiesteroide Desidrogenase Tipo 2
Tipo de estudo:
Observational_studies
Aspecto:
Determinantes_sociais_saude
Limite:
Adult
/
Female
/
Humans
/
Pregnancy
País/Região como assunto:
Asia
Idioma:
En
Revista:
J Obstet Gynaecol Res
Ano de publicação:
2015
Tipo de documento:
Article