Noncovalent fluorescent probes of human immuno- and constitutive proteasomes.
J Med Chem
; 57(21): 9211-7, 2014 Nov 13.
Article
em En
| MEDLINE
| ID: mdl-25333324
ABSTRACT
We report here the synthesis and biological evaluation of fluorescent probes functioning as inhibitors that noncovalently block human immuno- and constitutive proteasomes. These cell-penetrating linear analogues of the natural cyclopeptide TMC-95A were efficient on cells at the nanomolar level and assessed by confocal microscopy and flow cytometry. They may constitute an alternative to previously reported fluorescent probes that all bind covalently to proteasomes.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Complexo de Endopeptidases do Proteassoma
/
Inibidores de Proteassoma
/
Corantes Fluorescentes
Limite:
Humans
Idioma:
En
Revista:
J Med Chem
Ano de publicação:
2014
Tipo de documento:
Article