A novel chimeric amine dehydrogenase shows altered substrate specificity compared to its parent enzymes.
Chem Commun (Camb)
; 50(95): 14953-5, 2014 Dec 11.
Article
em En
| MEDLINE
| ID: mdl-25347124
ABSTRACT
We created a novel chimeric amine dehydrogenase (AmDH) via domain shuffling of two parent AmDHs ('L- and F-AmDH'), which in turn had been generated from leucine and phenylalanine DH, respectively. Unlike the parent proteins, the chimeric AmDH ('cFL-AmDH') catalyzes the amination of acetophenone to (R)-methylbenzylamine and adamantylmethylketone to adamantylethylamine.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas Recombinantes de Fusão
/
Leucina Desidrogenase
/
Aminoácido Oxirredutases
Idioma:
En
Revista:
Chem Commun (Camb)
Ano de publicação:
2014
Tipo de documento:
Article