Identification of suitable reference genes for gene expression studies by qRT-PCR in the blister beetle Mylabris cichorii.
J Insect Sci
; 14: 94, 2014.
Article
em En
| MEDLINE
| ID: mdl-25368050
ABSTRACT
The blister beetle Mylabris cichorii L. (Coleoptera Meloidae) is a traditional medicinal insect recorded in the Chinese Pharmacopoeia. It synthesizes cantharidin, which kills cancer cells efficiently. Only males produce large amounts of cantharidin. Reference genes are required as endogenous controls for the analysis of differential gene expression in M. cichorii. Our study chose 10 genes as candidate reference genes. The stability of expression of these genes was analyzed by quantitative PCR and determined with two algorithms, geNorm and Normfinder. We recommend UBE3A and RPL22e as suitable reference genes in females and UBE3A, TAF5, and RPL22e in males.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Besouros
/
Genes de Insetos
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
J Insect Sci
Ano de publicação:
2014
Tipo de documento:
Article