Endoplasmic reticulum stress inhibition is a valid therapeutic strategy in vitrifying oocytes.
Cryobiology
; 70(1): 48-52, 2015 Feb.
Article
em En
| MEDLINE
| ID: mdl-25499542
ABSTRACT
The aim of this study is to determine the link between oocyte cryopreservation and endoplasmic reticulum (ER) stress; whether ER stress inhibition improves the efficiency of oocyte vitrification is also explored. Oocytes from mice were exposure to tauroursodeoxycholic acid (TUDCA, an ER stress inhibitor) or TM (tunicamycin, an ER stress inducer) with or without vitrification. The expressions of X-box binding protein-1 (XBP-1) protein and caspase-12 protein, viability of vitrified-warmed oocytes, and their subsequent embryo competence were measured. The levels of XBP-1 protein and caspase-12 protein expression in vitrified-warmed oocytes were significantly higher than those of fresh control oocytes. TUDCA improved the viability of vitrified-warmed oocytes and their subsequent embryo competence. Mouse oocyte cryopreservation is associated with ER stress, and ER stress inhibition improves the efficiency of oocyte vitrification.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oócitos
/
Ácido Tauroquenodesoxicólico
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Tunicamicina
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Criopreservação
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Estresse do Retículo Endoplasmático
Limite:
Animals
Idioma:
En
Revista:
Cryobiology
Ano de publicação:
2015
Tipo de documento:
Article