Adventitial gene transfer of catalase attenuates angiotensin II-induced vascular remodeling.
Mol Med Rep
; 11(4): 2608-14, 2015 Apr.
Article
em En
| MEDLINE
| ID: mdl-25503998
ABSTRACT
Vascular adventitia and adventitiaderived reactive oxygen species (ROS) contribute to vascular remodeling following vascular injury. A previous ex vivo study in adventitial fibroblasts showed that catalase, one of most important antioxide enzymes, was downregulated by angiotensin II (AngII). The aim of the present study was to investigate whether adventitial gene transfer of catalase affects AngIIinduced vascular remodeling in vivo. Adenoviruses coexpressing catalase and enhanced green fluorescent protein (eGFP) or expressing eGFP only were applied to the adventitial surface of common carotid arteries of SpragueDawley rats. Alzet minipumps administering AngII (0.75 mg/kg/day) were then implanted subcutaneously for 14 days. Systolic blood pressure and biological parameters of vascular remodeling were measured in each group. Adventitial fibroblasts were cultured and p38 mitogenactivated protein kinase (MAPK) phosphorylation was measured using western blot analysis. The results showed that adventitial gene transfer of catalase had no effect on AngIIinduced systolic blood pressure elevation. However, catalase adenovirus transfection significantly inhibited AngIIinduced media hypertrophy compared with that of the control virus (P<0.05). In addition, catalase transfection significantly attenuated AngIIinduced ROS generation, macrophage infiltration, collagen deposition and adventitial αsmooth muscle actin expression. Furthermore, catalase transfection significantly inhibited the AngIIinduced increase in p38MAPK phosphorylation. In conclusion, the results of the present study demonstrated that adventitial gene transfer of catalase significantly attenuated AngIIinduced vascular remodeling in rats via inhibition of adventitial p38MAPK phosphorylation.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Angiotensina II
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Catalase
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Técnicas de Transferência de Genes
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Túnica Adventícia
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Remodelação Vascular
Limite:
Animals
Idioma:
En
Revista:
Mol Med Rep
Ano de publicação:
2015
Tipo de documento:
Article