[Effect of fluoride on the viability and apoptosis of ameloblasts in vitro].
Shanghai Kou Qiang Yi Xue
; 23(5): 519-23, 2014 Oct.
Article
em Zh
| MEDLINE
| ID: mdl-25543592
ABSTRACT
PURPOSE:
To evaluate the effect of fluoride on viability of rat ameloblasts in vitro.METHODS:
The ameloblasts of rat was exposed to different concentrations of NaF (0, 0.4, 0.8, 1.6, 3.2, 6.4 mmol/L) for 24, 48 and 72 hours. CCK-8 assays were performed to measure the cells proliferation; The morphology of apoptosis was observed by Hoechst 33258 staining and the rate of apoptosis was determined by flow cytometry. The data was analyzed using SPSS 13.0 software package.RESULTS:
(1)The proliferation of ameloblasts was increased when concentrations of NaF between 0.4 mmol/L and 0.8 mmol/L, whereas inhibited at 1.6 mmol/L NaF and above. The effects were in time-dependent manner.(2)Cells in the 1.6 mmol/L NaF groups showed unclear karyorrhexis and apoptotic cell morphology. The effects were in concentration-dependent manner.CONCLUSIONS:
(1)Fluoride has two-phase effects to ameloblasts At low doses, it promoted cell proliferation while at high doses it had negative effects. (2)1.6 mmol/L NaF could induce apoptosis of ameloblasts.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fluoreto de Sódio
/
Ameloblastos
Limite:
Animals
Idioma:
Zh
Revista:
Shanghai Kou Qiang Yi Xue
Ano de publicação:
2014
Tipo de documento:
Article