Overexpression of miR145 in U87 cells reduces glioma cell malignant phenotype and promotes survival after in vivo implantation.
Int J Oncol
; 46(3): 1031-8, 2015 Mar.
Article
em En
| MEDLINE
| ID: mdl-25544346
ABSTRACT
In the present study, we sought to elucidate the effect of miR145 on glioma cell progression and its mechanisms of action. We examined the effects of miR145 on proliferation and invasion of U87 glioma cells and on capillary tube formation. Our data show that restoration of miR145 in U87 glioma cells significantly reduced their in vitro proliferation, invasion and angiogenesis. However, decreased miR145 expression promoted U87 glioma cell proliferation, invasion and angiogenesis, and reduced-expression of miR145 increased ADAM17 and EGFR expression in U87 cells. Overexpression of miR145 reduced ADAM17 and EGFR expression. VEGF secretion and VEGF expression were decreased by increased miR145 expression in U87 cells and were reversed by miR145 downregulation in vitro. Nude mice with intracerebral implantation of U87 overexpressing miR145 cells exhibited significantly reduced tumor growth and promoted survival compared with control groups. Taken together, these results suggest a role for miR145 as a tumor suppressor which inhibits glioma cell proliferation, invasion and angiogenesis in vitro and reduces glioma growth in vivo.
Texto completo:
1
Coleções:
01-internacional
Contexto em Saúde:
6_ODS3_enfermedades_notrasmisibles
Base de dados:
MEDLINE
Assunto principal:
Regulação Neoplásica da Expressão Gênica
/
MicroRNAs
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Glioma
Tipo de estudo:
Prognostic_studies
Limite:
Animals
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Humans
Idioma:
En
Revista:
Int J Oncol
Ano de publicação:
2015
Tipo de documento:
Article