Production of cell-penetrating peptides in Escherichia coli using an intein-mediated system.
Appl Biochem Biotechnol
; 175(6): 3025-37, 2015 Mar.
Article
em En
| MEDLINE
| ID: mdl-25586490
ABSTRACT
Cell-penetrating peptides are molecules with the ability to cross membranes and enter cells. Attention has been put on these peptides as a tool for drug delivery research, as they are able to serve as delivery vectors for large molecules. Intracellular delivery of bioactive peptides is a very promising research area for clinical applications, since peptides are able to simulate protein regions and thus modulate key intracellular protein-protein interactions. Therefore, evaluation of different strategies for production of these peptides is necessary. In this work, an intein-mediated system was used to evaluate Escherichia coli recombinant production of p53pAnt and PNC27 anticancer cell-penetrating peptides. It was demonstrated that the pTXB1 and the pTYB11 vector systems are suitable for production of this kind of peptides. The production process involves a low-temperature induction process and an efficient on-column intein-mediated cleavage, which allowed an effective peptide recovery using a single chromatographic step.
Texto completo:
1
Coleções:
01-internacional
Contexto em Saúde:
3_ND
Base de dados:
MEDLINE
Assunto principal:
Inteínas
/
Escherichia coli
/
Peptídeos Penetradores de Células
Idioma:
En
Revista:
Appl Biochem Biotechnol
Ano de publicação:
2015
Tipo de documento:
Article