Experimental infection of Z:ZCLA Mongolian gerbils with human hepatitis E virus.
World J Gastroenterol
; 21(3): 862-7, 2015 Jan 21.
Article
em En
| MEDLINE
| ID: mdl-25624719
ABSTRACT
AIM:
To investigate whether ZZCLA Mongolian gerbils are readily susceptible to infection by human hepatitis E virus (HEV).METHODS:
ZZCLA Mongolian gerbils were infected with a clinical HEV strain isolated from an acute hepatitis E patient, and virus pathogenesis was assessed in this host. Non-infected gerbils served as the control group. Feces samples from gerbils were collected weekly for reverse transcription-nested polymerase chain reaction. Serum anti-HEV IgG and alanine aminotransferase (ALT) were detected by enzyme linked immunosorbent assay. At sacrifice, each animal's liver, spleen and kidney were collected for histopathologic examination.RESULTS:
HEV-infected gerbils showed fatigue, with histopathological changes observed in the liver, spleen and kidney. HEV RNA was detected in fecal samples taken at day 7 after inoculation and the detectable levels lasted out to day 42 after inoculation. Interestingly, ALT levels were only moderately increased in the HEV-infected animals compared with the non-infected control group.CONCLUSION:
ZZCLA Mongolian gerbils are susceptible to human HEV.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Contexto em Saúde:
2_ODS3
Base de dados:
MEDLINE
Assunto principal:
Vírus da Hepatite E
/
Hepatite E
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Hepatite Viral Animal
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Animals
/
Humans
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Male
Idioma:
En
Revista:
World J Gastroenterol
Ano de publicação:
2015
Tipo de documento:
Article