Deep resequencing of mitochondrial DNA.
Methods Mol Biol
; 1264: 59-66, 2015.
Article
em En
| MEDLINE
| ID: mdl-25631003
ABSTRACT
Detecting and quantifying low-level variants in mitochondrial DNA (mtDNA) by deep resequencing can lead to important insights into the biology of mtDNA in health and disease. Massively parallel ("next-generation") sequencing is an attractive tool owing to the great depth and breadth of coverage. However, there are several important challenges to be considered when using this method, in particular the avoidance of false discovery due to the unintended amplification of nuclear pseudogenes and the approach to delineating signal from noise at very great depths of coverage. Here we present methods for whole mtDNA genome deep sequencing (Illumina MiSeq) and short amplicon deep sequencing (Roche 454 GS-FLX).
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA Mitocondrial
/
Sequenciamento de Nucleotídeos em Larga Escala
Idioma:
En
Revista:
Methods Mol Biol
Ano de publicação:
2015
Tipo de documento:
Article