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Microcontroller-assisted compensation of adenosine triphosphate levels: instrument and method development.
Hu, Jie-Bi; Chen, Ting-Ru; Chen, Yu-Chie; Urban, Pawel L.
Afiliação
  • Hu JB; Department of Applied Chemistry, National Chiao Tung University, 1001 University Rd, Hsinchu, 300, Taiwan.
  • Chen TR; Department of Applied Chemistry, National Chiao Tung University, 1001 University Rd, Hsinchu, 300, Taiwan.
  • Chen YC; 1] Department of Applied Chemistry, National Chiao Tung University, 1001 University Rd, Hsinchu, 300, Taiwan [2] Institute of Molecular Science, National Chiao Tung University, 1001 University Rd, Hsinchu, 300, Taiwan.
  • Urban PL; 1] Department of Applied Chemistry, National Chiao Tung University, 1001 University Rd, Hsinchu, 300, Taiwan [2] Institute of Molecular Science, National Chiao Tung University, 1001 University Rd, Hsinchu, 300, Taiwan.
Sci Rep ; 5: 8135, 2015 Jan 30.
Article em En | MEDLINE | ID: mdl-25633338
ABSTRACT
In order to ascertain optimum conditions for biocatalytic processes carried out in vitro, we have designed a bio-opto-electronic system which ensures real-time compensation for depletion of adenosine triphosphate (ATP) in reactions involving transfer of phosphate groups. The system covers ATP concentration range of 2-48 µM. The report demonstrates feasibility of the device operation using apyrase as the ATP-depleting enzyme.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Trifosfato de Adenosina / Microtecnologia Idioma: En Revista: Sci Rep Ano de publicação: 2015 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Trifosfato de Adenosina / Microtecnologia Idioma: En Revista: Sci Rep Ano de publicação: 2015 Tipo de documento: Article