Expression profile and potential roles of EVA1A in normal and neoplastic pancreatic tissues.
Asian Pac J Cancer Prev
; 16(1): 373-6, 2015.
Article
em En
| MEDLINE
| ID: mdl-25640383
ABSTRACT
BACKGROUND:
EVA1A (eva-1 homolog A) is a novel gene that regulates programmed cell death through autophagy and apoptosis. Our objective was to investigate the expression profiles and potential role of EVA1A in normal and neoplastic human pancreatic tissues. MATERIALS ANDMETHODS:
The expression pattern of EVA1A in normal pancreatic tissue was examined by indirect immunofluorescence and confocal microscopy. Protein levels in paraffin-embedded specimens from normal and diseased pancreatic and matched non-tumor tissues were evaluated by immunohistochemistry.RESULTS:
EVA1A colocalized with glucagon but not with insulin, demonstrating production in islet alpha cells. Itwas strongly expressed in chronic pancreatitis, moderately or weakly expressed in the plasma membrane and cytoplasm in pancreatic acinar cell carcinoma, and absent in normal pancreatic acinar cells. Although the tissue architecture was deformed, EVA1A was absent in the alpha cells of pancreatic ductal adenocarcinomas, intraductal papillary mucinous neoplasms, mucinous cystadenomas, solid papillary tumors and pancreatic neuroendocrine tumors.CONCLUSIONS:
EVA1A protein is specifically expressed in islet alpha cells, suggesting it may play an important role in regulating alpha-cell function. The ectopic expression of EVA1A in pancreatic neoplasms may contribute to their pathogenesis and warrants further investigation.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Neoplasias Pancreáticas
/
Pancreatite
/
Carcinoma de Células Acinares
/
Carcinoma Ductal Pancreático
/
Proteínas de Membrana
Limite:
Humans
Idioma:
En
Revista:
Asian Pac J Cancer Prev
Ano de publicação:
2015
Tipo de documento:
Article