Prevalence of rotavirus antibodies in breast milk and inhibitory effects to rotavirus vaccines.
Hum Vaccin Immunother
; 10(12): 3681-7, 2014.
Article
em En
| MEDLINE
| ID: mdl-25668672
ABSTRACT
Rotavirus (RV) is the most common cause of childhood diarrhea worldwide, and several vaccines have been successfully developed to reduce the burden of disease. However, lower vaccine immunogenicity and efficacy in developing countries might be related to the virus-neutralizing activity of breast milk. We examined possible differences in breast milk antibody levels (total IgA antibody, RV-specific antibodies, and RV-neutralizing antibodies) between healthy mothers living in a rural area (n=145) and mothers living in an urban area (n=147) of Vietnam. Total IgA concentration was significantly higher in samples from mothers in the rural region than in samples from mothers in the urban region, whereas urban mothers had significantly higher RV-specific IgA antibody titers than did rural mothers. Neutralizing antibodies against RV strain G1P[8] were undetected in nearly one-half of the breast milk samples (45-48%), whereas the majority of the remaining samples had low antibody titers (2-16). Despite these low titers, the breast milk still reduced vaccine strain titers (2×10(6) plaque forming units/mL) up to 80% or more, even at a milk-to-virus ratio of 18. An increase in neutralizing anti-G1P[8] antibody titers (P<0.05) in rural infants over time suggests a continuous exposure to circulating RV. These results contribute to the understanding of the potential interference of breast milk with RV vaccine efficacy and immunogenicity in Vietnamese infants.
Palavras-chave
DTP, diphtheriatetanuspertussis; ELISA, Enzyme linked immunosorbent assay; G1-VN, neutralizing antibodies against G1P[8]; G4-VN, neutralizing antibodies against G4P[6]; HCl, Hydrogen chloride; HRP, Horseradish peroxidase; IgA; IgA, Immunoglobulin A; IgG, Immunoglobulin G; MEM, Minimal essential medium; NIHE, National Institute of Hygiene and Epidemiology; OD, Optical density; P-RV, Rotavirus strain P; PBS, Phosphate-buffered saline; PFU, Plaque-forming unit; POLYVAC, Center for Production of Vaccines and Biologicals; RRV, Rhesus rotavirus; RV, Rotavirus; SE, Standard error;; TMB, 3, 3', 5, 5'-Tetramethylbenzidine; Vietnam; breast milk; neutralizing antibody; rotavirus
Texto completo:
1
Coleções:
01-internacional
Contexto em Saúde:
3_ND
Base de dados:
MEDLINE
Assunto principal:
Rotavirus
/
Vacinas contra Rotavirus
/
Leite Humano
/
Anticorpos Antivirais
Tipo de estudo:
Observational_studies
/
Prevalence_studies
/
Risk_factors_studies
Limite:
Adolescent
/
Adult
/
Female
/
Humans
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Infant
/
Middle aged
Idioma:
En
Revista:
Hum Vaccin Immunother
Ano de publicação:
2014
Tipo de documento:
Article