Autofagia; Chaperonas Moleculares/química; Fragmentos de Peptídeos/química; Fosfopeptídeos/química; Ribonucleoproteína Nuclear Pequena U1/química; Animais; Autoimunidade; Linfócitos B/imunologia; Endocitose; Endossomos/metabolismo; Proteínas de Choque Térmico HSC70/química; Antígenos de Histocompatibilidade Classe II/metabolismo; Humanos; Lúpus Eritematoso Sistêmico/imunologia; Lisossomos/química; Lisossomos/metabolismo; Camundongos; Camundongos Endogâmicos MRL lpr; Células NIH 3T3; Fosforilação; Serina/química; Linfócitos T/imunologia

ALF, artificial lysosomal fluid; APC, antigen-presenting cell; B lymphocytes; CMA, chaperone-mediated autophagy; CPZ: chlorpromazine; CTSD, cathepsin D; CoIP, coimmunoprecipitation; DAPI, 4', 6-diamidino-2-phenylindole; ELISA, enzyme-linked immunosorbent assay; FCS, fetal calf serum; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; HCQ, hydroxychloroquine; HSPA8/HSC70; LAMP2A, lysosomal-associated membrane protein 2A; LC-MS, liquid chromatography-mass spectrometry; LC3-II, MAP1LC3-II; MHCII, major histocompatibility complex class II; NBD, nucleotide binding domain; PBS, phosphate-buffered saline; RP-HPLC, reversed-phase high-performance liquid chromatography; RPL5, ribosomal protein L5; SBD, substrate binding domain; SD, standard deviation; SEM, standard error of the mean; SLE, systemic lupus erythematosus; SNRNP70/U170K: small nuclear ribonucleoprotein 70kDa; SQSTM1/p62, sequestosome 1; TF, transferrin; TFA, trifluoroacetic acid; antigen-presenting cells; autophagy; bodipy: BODIPY FL C5 Lactosylceramide/bovine serum albumin; chaperone-mediated autophagy; class II MHC molecules; heat shock proteins; iv, intravenous; lupus; lysosomal chaperones; lysosomes; paraquat, 1, 1'-dimethyl-4, 4'-bipyridyldinium dichloride; qRT-PCR, quantitative reverse transcriptase-polymerase chain reaction