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Genetic analysis of seed-shattering genes in rice using an F3:4 population derived from an Oryza sativa x Oryza rufipogon cross.
Kwon, S-J; Yu, J; Park, Y J; Son, J-H; Kim, N-S; Lee, J K.
Afiliação
  • Kwon SJ; Department of Plant Resources, College of Industrial Science, Kongju National University, Yesan, Korea.
  • Yu J; Department of Plant Resources, College of Industrial Science, Kongju National University, Yesan, Korea.
  • Park YJ; Department of Plant Resources, College of Industrial Science, Kongju National University, Yesan, Korea.
  • Son JH; Department of Molecular Bioscience, Institute of Bioscience and Biotechnology, Kangwon National University, Chuncheon, Korea.
  • Kim NS; Department of Molecular Bioscience, Institute of Bioscience and Biotechnology, Kangwon National University, Chuncheon, Korea.
  • Lee JK; Department of Applied Plant Sciences, Oriental Bio-Herb Research Institute, Kangwon National University, Chuncheon, Korea jukyonglee@kangwon.ac.kr.
Genet Mol Res ; 14(1): 1347-61, 2015 Feb 13.
Article em En | MEDLINE | ID: mdl-25730074
Seed shattering of wild plant species is thought to be an adaptive trait to facilitate seed dispersal. For rice breeding, seed shatter-ing is an important trait for improving breeding strategies, particularly when developing lines use interspecific hybrids and introgression of genes from wild species. We developed F3:4 recombinant inbred lines from an interspecific cross between Oryza sativa cv. Ilpoombyeo and Oryza rufipogon. In this study, we genetically analyzed known shat-tering-related loci using the F3:4 population of O. sativa/O. rufipogon. CACTA-AG190 was significantly associated with the shattering trait CACTA-TD according to bulked segregant analysis results, and was found in the qSH-1 region of chromosome 1. Fine genetic mapping of the flanking regions around qSH-1 based on CACTA-AG190 revealed multiple-sequence variations. The highest limit of detection based on quantitative trait locus analysis was observed between shaap-7715 and a 518-bp insertion site. Two other quantitative trait locus analyses of seed-shattering-related loci, qSH-4 and sh-h, were performed using simple sequence repeat and allele-pecific single nucleotide polymor-phism markers. Our results can be applied for rice-breeding research, such as marker-assisted selection between cultivated and wild rice.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oryza / Sementes / Genes de Plantas Tipo de estudo: Prognostic_studies Idioma: En Revista: Genet Mol Res Ano de publicação: 2015 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oryza / Sementes / Genes de Plantas Tipo de estudo: Prognostic_studies Idioma: En Revista: Genet Mol Res Ano de publicação: 2015 Tipo de documento: Article