Cell density dependence of transformation frequencies in C3H10T1/2 cells exposed to X-rays.
Int J Radiat Biol
; 56(6): 989-98, 1989 Dec.
Article
em En
| MEDLINE
| ID: mdl-2574226
ABSTRACT
The effects of cell density on transformation frequencies were studied in C3H10T1/2 cells exposed to 0.5 and 7 Gy of 200 kVp X-rays. Initial cell density strongly influenced transformation frequency; this decreased by a factor of between 4 and 10 when the initial seeding density was changed from 50 to 2500 cells/10 cm diameter Petri dish. The data were fitted with two equations (a) an allometric function represented on a log-log scale by a straight line and (b) a sigmoidal function with plateaux between 50 and 250 cells/dish and above 600. The two curves are compared and their probabilities discussed. Our data indicate that the region between 50 and 250 cells/dish would be the most suitable region for dose-effect measurements. A study of the growth curves at 0.5 and 8.5 Gy shows that cell growth rates are not influenced by initial cell density.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Transformação Celular Neoplásica
Limite:
Animals
Idioma:
En
Revista:
Int J Radiat Biol
Ano de publicação:
1989
Tipo de documento:
Article