Rapid evaluation of 25 key sphingolipids and phosphosphingolipids in human plasma by LC-MS/MS.
Anal Bioanal Chem
; 407(17): 5189-98, 2015 Jul.
Article
em En
| MEDLINE
| ID: mdl-25749796
ABSTRACT
We report on a new, sensitive, and fast LC-MS/MS method for the simultaneous determination of 25 key sphingolipid components in human plasma, including phosphorylated sphinganine and sphingosine, in a single 9-min run. This method enables an effective and high-throughput coverage of the metabolic changes involving the sphingolipidome during physiological or pathological states. The method is based on liquid-liquid extraction followed by reversed-phase LC-MS/MS. Exogenous odd-chain lipids are used as cost-effective but reliable internal standards. The method was fully validated in surrogate matrix and naive human plasma following FDA guidelines. Sample stability and dilution integrity were also tested and verified.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fosfolipídeos
/
Esfingolipídeos
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Cromatografia Líquida de Alta Pressão
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Espectrometria de Massas em Tandem
Limite:
Female
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Humans
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Male
Idioma:
En
Revista:
Anal Bioanal Chem
Ano de publicação:
2015
Tipo de documento:
Article