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Stem cells from human amniotic fluid exert immunoregulatory function via secreted indoleamine 2,3-dioxygenase1.
Romani, Rita; Pirisinu, Irene; Calvitti, Mario; Pallotta, Maria Teresa; Gargaro, Marco; Bistoni, Giovanni; Vacca, Carmine; Di Michele, Alessandro; Orabona, Ciriana; Rosati, Jessica; Pirro, Matteo; Giovagnoli, Stefano; Matino, Davide; Prontera, Paolo; Rosi, Gabriella; Grohmann, Ursula; Talesa, Vincenzo N; Donti, Emilio; Puccetti, Paolo; Fallarino, Francesca.
Afiliação
  • Romani R; Department of Experimental Medicine, University of Perugia, Perugia, Italy.
  • Pirisinu I; Department of Experimental Medicine, University of Perugia, Perugia, Italy.
  • Calvitti M; Department of Experimental Medicine, University of Perugia, Perugia, Italy.
  • Pallotta MT; Department of Experimental Medicine, University of Perugia, Perugia, Italy.
  • Gargaro M; Department of Experimental Medicine, University of Perugia, Perugia, Italy.
  • Bistoni G; Plastic Surgery Unit, Hospital Universitario de la Ribera, Valencia, Spain.
  • Vacca C; Department of Surgery, 'La Sapienza' University, Rome, Italy.
  • Di Michele A; Department of Experimental Medicine, University of Perugia, Perugia, Italy.
  • Orabona C; Department of Physics and Geology, University of Perugia, Perugia, Italy.
  • Rosati J; Department of Experimental Medicine, University of Perugia, Perugia, Italy.
  • Pirro M; iPS-Cellular Reprogramming Unit, Fondazione Casa Sollievo della Sofferenza, Mendel, Rome, Italy.
  • Giovagnoli S; Department of Medicine, University of Perugia, Perugia, Italy.
  • Matino D; Department of Pharmaceutical Sciences, University of Perugia, Perugia, Italy.
  • Prontera P; Department of Experimental Medicine, University of Perugia, Perugia, Italy.
  • Rosi G; Department of Surgery and Biomedical Sciences, University of Perugia, Perugia, Italy.
  • Grohmann U; Department of Experimental Medicine, University of Perugia, Perugia, Italy.
  • Talesa VN; Department of Experimental Medicine, University of Perugia, Perugia, Italy.
  • Donti E; Department of Experimental Medicine, University of Perugia, Perugia, Italy.
  • Puccetti P; Department of Surgery and Biomedical Sciences, University of Perugia, Perugia, Italy.
  • Fallarino F; Department of Experimental Medicine, University of Perugia, Perugia, Italy.
J Cell Mol Med ; 19(7): 1593-605, 2015 Jul.
Article em En | MEDLINE | ID: mdl-25783564
Although human amniotic fluid does contain different populations of foetal-derived stem cells, scanty information is available on the stemness and the potential immunomodulatory activity of in vitro expanded, amniotic fluid stem cells. By means of a methodology unrequiring immune selection, we isolated and characterized different stem cell types from second-trimester human amniotic fluid samples (human amniotic fluid stem cells, HASCs). Of those populations, one was characterized by a fast doubling time, and cells were thus designated as fHASCs. Cells maintained their original phenotype under prolonged in vitro passaging, and they were able to originate embryoid bodies. Moreover, fHASCs exhibited regulatory properties when treated with interferon (IFN)-γ, including induction of the immunomodulatory enzyme indoleamine 2,3-dioxygenase 1 (IDO1). On coculture with human peripheral blood mononuclear cells, IFN-γ-treated fHASCs caused significantly decreased T-cell proliferation and increased frequency in CD4(+)  CD25(+)  FOXP3(+) regulatory T cells. Both effects required an intact IDO1 function and were cell contact-independent. An unprecedented finding in our study was that purified vesicles from IFN-γ-treated fHASCs abundantly expressed the functional IDO1 protein, and those vesicles were endowed with an fHASC-like regulatory function. In vivo, fHASCs were capable of immunoregulatory function, promoting allograft survival in a mouse model of allogeneic skin transplantation. This was concurrent with the expansion of CD4(+)  CD25(+)  Foxp3(+) T cells in graft-draining lymph nodes from recipient mice. Thus fHASCs, or vesicles thereof, may represent a novel opportunity for immunoregulatory maneuvers both in vitro and in vivo.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Células-Tronco / Indolamina-Pirrol 2,3,-Dioxigenase / Imunomodulação / Líquido Amniótico Tipo de estudo: Prognostic_studies Limite: Adult / Animals / Humans Idioma: En Revista: J Cell Mol Med Ano de publicação: 2015 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Células-Tronco / Indolamina-Pirrol 2,3,-Dioxigenase / Imunomodulação / Líquido Amniótico Tipo de estudo: Prognostic_studies Limite: Adult / Animals / Humans Idioma: En Revista: J Cell Mol Med Ano de publicação: 2015 Tipo de documento: Article