Ultrasound-modulated fluorescence based on donor-acceptor-labeled microbubbles.
J Biomed Opt
; 20(3): 036012, 2015 03.
Article
em En
| MEDLINE
| ID: mdl-25789423
ABSTRACT
A fluorescence resonance energy transfer (FRET)-based microbubble contrast agent system was designed to experimentally demonstrate the concept of ultrasound-modulated fluorescence (UMF). Microbubbles were simultaneously labeled with donor and acceptor fluorophores on the surface to minimize self-quenching and maximize FRET. In response to ultrasound, the quenching efficiency was greatly modulated by changing the distance between the donor and acceptor molecules through microbubble size oscillations. Both donors and acceptors exhibited UMF on individual microbubbles. The UMF strength of the donor was more significant compared to that of the acceptor. Furthermore, the UMF of the donor was observed from a microbubble solution in a turbid media. This study exploits the feasibility of donoracceptor labeled microbubbles as UMF contrast agents.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ultrassonografia
/
Meios de Contraste
/
Transferência Ressonante de Energia de Fluorescência
/
Microbolhas
/
Corantes Fluorescentes
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
J Biomed Opt
Ano de publicação:
2015
Tipo de documento:
Article