Suppression of murine IgE responses with amino acid polymer/allergen conjugates. I. Preparation of poly-(N-methylglycine)/grass pollen extract conjugates using 4-(methylmercapto)phenyl-succinimidyl succinate as coupling reagent.
Int Arch Allergy Appl Immunol
; 76(4): 354-60, 1985.
Article
em En
| MEDLINE
| ID: mdl-2579914
ABSTRACT
A procedure utilising the latent activating potential of the 4-(methylmercapto)phenyl ester group has been developed for the controlled, reproducible preparation of macromolecular conjugates. This ester, as part of a succinyl-bridging group, was used to couple the water-soluble, amino acid polymer, poly-(N-methylglycine) (polysarcosine), via its N-terminal secondary amine, with the nucleophilic components of the aqueous extract of a mixture of grass pollens. The products exhibit a reproducible, antigen-specific suppressive effect on the in vivo synthesis of IgE in mice.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Peptídeos
/
Sarcosina
/
Succinimidas
/
Imunoglobulina E
/
Alérgenos
Limite:
Animals
Idioma:
En
Revista:
Int Arch Allergy Appl Immunol
Ano de publicação:
1985
Tipo de documento:
Article