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Associated bacteria of different life stages of Meloidogyne incognita using pyrosequencing-based analysis.
Cao, Yi; Tian, Baoyu; Ji, Xinglai; Shang, Shenghua; Lu, Chaojun; Zhang, Keqin.
Afiliação
  • Cao Y; Key Laboratory for Conservation and Utilization of Bio-resource, and Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan University, Kunming, China.
  • Tian B; Key Laboratory of Molecular Genetics, Guizhou Academy of Tobacco Science, Guiyang, China.
  • Ji X; College of Life Science, Fujian Normal University, Fuzhou, China.
  • Shang S; Key Laboratory for Conservation and Utilization of Bio-resource, and Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan University, Kunming, China.
  • Lu C; Key Laboratory of Molecular Genetics, Guizhou Academy of Tobacco Science, Guiyang, China.
  • Zhang K; Key Laboratory for Conservation and Utilization of Bio-resource, and Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan University, Kunming, China.
J Basic Microbiol ; 55(8): 950-60, 2015 Aug.
Article em En | MEDLINE | ID: mdl-25809195
The root knot nematode (RKN), Meloidogyne incognita, belongs to the most damaging plant pathogens worldwide, and is able to infect almost all cultivated plants, like tomato. Recent research supports the hypothesis that bacteria often associated with plant-parasitic nematodes, function as nematode parasites, symbionts, or commensal organisms etc. In this study, we explored the bacterial consortia associated with M. incognita at different developmental stages, including egg mass, adult female and second-stage juvenile using the pyrosequencing approach. The results showed that Proteobacteria, with a proportion of 71-84%, is the most abundant phylum associated with M. incognita in infected tomato roots, followed by Actinobacteria, Bacteroidetes, Firmicutes etc. Egg mass, female and second-stage juvenile of M. incognita harbored a core microbiome with minor difference in communities and diversities. Several bacteria genera identified in M. incognita are recognized cellulosic microorganisms, pathogenic bacteria, nitrogen-fixing bacteria and antagonists to M. incognita. Some genera previously identified in other plant-parasitic nematodes were also found in tomato RKNs. The potential biological control microorganisms, including the known bacterial pathogens and nematode antagonists, such as Actinomycetes and Pseudomonas, showed the largest diversity and proportion in egg mass, and dramatically decreased in second-stage juvenile and female of M. incognita. This is the first comprehensive report of bacterial flora associated with the RKN identified by pyrosequencing-based analysis. The results provide valuable information for understanding nematode-microbiota interactions and may be helpful in the development of novel nematode-control strategies.
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Texto completo: 1 Coleções: 01-internacional Contexto em Saúde: 3_ND Base de dados: MEDLINE Assunto principal: Bactérias / Tylenchoidea / Raízes de Plantas / Consórcios Microbianos / Estágios do Ciclo de Vida Tipo de estudo: Prognostic_studies / Risk_factors_studies Limite: Animals Idioma: En Revista: J Basic Microbiol Ano de publicação: 2015 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Contexto em Saúde: 3_ND Base de dados: MEDLINE Assunto principal: Bactérias / Tylenchoidea / Raízes de Plantas / Consórcios Microbianos / Estágios do Ciclo de Vida Tipo de estudo: Prognostic_studies / Risk_factors_studies Limite: Animals Idioma: En Revista: J Basic Microbiol Ano de publicação: 2015 Tipo de documento: Article