Calcium silicate/calcium phosphate biphasic cements for vital pulp therapy: chemical-physical properties and human pulp cells response.
Clin Oral Investig
; 19(8): 2075-89, 2015 Nov.
Article
em En
| MEDLINE
| ID: mdl-25820989
ABSTRACT
OBJECTIVES:
The aim was to test the properties of experimental calcium silicate/calcium phosphate biphasic cements with hydraulic properties designed for vital pulp therapy as direct pulp cap and pulpotomy.METHODS:
CaSi-αTCP and CaSi-DCDP were tested for ion-releasing ability, solubility, water sorption, porosity, ability to nucleate calcium phosphates, and odontoblastic differentiationalkaline phosphatase (ALP) and osteocalcin (OCN) upregulationof primary human dental pulp cells (HDPCs).RESULTS:
The materials showed high Ca and OH release, high open pore volume and apparent porosity, and a pronounced ability to nucleate calcium phosphates on their surface. HDPCs treated with CaSi-αTCP showed a strong upregulation of ALP and OCN genes, namely a tenfold increase for OCN and a threefold increase for ALP compared to the control cells. Conversely, CaSi-DCDP induced a pronounced OCN gene upregulation but had no effect on ALP gene regulation.CONCLUSIONS:
Both cements showed high biointeractivity (release of Ca and OH ions) correlated with their marked ability to nucleate calcium phosphates. CaSi-αTCP cement proved to be a potent inducer of ALP and OCN genes as characteristic markers of mineralization processes normally poorly expressed by HDPCs. CLINICAL RELEVANCE Calcium silicate/calcium phosphate cements appear to be attractive new materials for vital pulp therapy as they may provide odontogenic/dentinogenic chemical signals for pulp regeneration and healing, and dentin formation in regenerative endodontics.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Teste de Materiais
/
Fosfatos de Cálcio
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Silicatos
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Compostos de Cálcio
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Cimentos Dentários
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Polpa Dentária
Limite:
Humans
Idioma:
En
Revista:
Clin Oral Investig
Ano de publicação:
2015
Tipo de documento:
Article