Repair of osteochondral defects in the minipig model by OPF hydrogel loaded with adipose-derived mesenchymal stem cells.
Regen Med
; 10(2): 135-51, 2015.
Article
em En
| MEDLINE
| ID: mdl-25835479
ABSTRACT
AIM:
Critical knee osteochondral defects in seven adult minipigs were treated with oligo(polyethylene glycol)fumarate (OPF) hydrogel combined with autologous or human adipose-derived stem cells (ASCs), and evaluated after 6 months.METHODS:
Four defects were made on the peripheral part of right trochleas (n = 28), and treated with OPF scaffold alone or pre-seeded with ASCs.RESULTS:
A better quality cartilage tissue characterized by improved biomechanical properties and higher collagen type II expression was observed in the defects treated by autologous or human ASC-loaded OPF; similarly this approach induced the regeneration of more mature bone with upregulation of collagen type I expression.CONCLUSION:
This study provides the evidence that both porcine and human adipose-derived stem cells associated to OPF hydrogel allow improving osteochondral defect regeneration in a minipig model.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Adipócitos
/
Hidrogéis
/
Engenharia Tecidual
/
Células-Tronco Mesenquimais
Limite:
Animals
/
Humans
/
Male
Idioma:
En
Revista:
Regen Med
Ano de publicação:
2015
Tipo de documento:
Article