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Axonal amphoterin mRNA is regulated by translational control and enhances axon outgrowth.
Merianda, Tanuja T; Coleman, Jennifer; Kim, Hak Hee; Kumar Sahoo, Pabitra; Gomes, Cynthia; Brito-Vargas, Paul; Rauvala, Heikki; Blesch, Armin; Yoo, Soonmoon; Twiss, Jeffery L.
Afiliação
  • Merianda TT; Department of Anatomy and Neurobiology, Drexel University College of Medicine, Philadelphia, Pennsylvania 19129.
  • Coleman J; Nemours Biomedical Research, Alfred I. duPont Hospital for Children, Wilmington, Delaware 19803.
  • Kim HH; Nemours Biomedical Research, Alfred I. duPont Hospital for Children, Wilmington, Delaware 19803.
  • Kumar Sahoo P; Department of Biological Sciences, University of South Carolina, Columbia, South Carolina 29208.
  • Gomes C; Department of Biological Sciences, University of South Carolina, Columbia, South Carolina 29208.
  • Brito-Vargas P; Department of Biological Sciences, University of South Carolina, Columbia, South Carolina 29208.
  • Rauvala H; Neuroscience Center, University of Helsinki, 00790 Helsinki, Finland.
  • Blesch A; Laboratory for Neuroregeneration, Spinal Cord Injury Center, Heidelberg University Hospital, 69118 Heidelberg, Germany and.
  • Yoo S; Nemours Biomedical Research, Alfred I. duPont Hospital for Children, Wilmington, Delaware 19803.
  • Twiss JL; Department of Anatomy and Neurobiology, Drexel University College of Medicine, Philadelphia, Pennsylvania 19129, Department of Biological Sciences, University of South Carolina, Columbia, South Carolina 29208, twiss@mailbox.sc.edu.
J Neurosci ; 35(14): 5693-706, 2015 Apr 08.
Article em En | MEDLINE | ID: mdl-25855182
ABSTRACT
High mobility group (HMG) proteins concentrate in the nucleus, interacting with chromatin. Amphoterin is an HMG protein (HMGB1) that has been shown to have extranuclear functions and can be secreted from some cell types. Exogenous amphoterin can increase neurite growth, suggesting that the secreted protein may have growth promoting activities in neurons. Consistent with this, we show that depletion of amphoterin mRNA from cultured adult rat DRG neurons attenuates neurite outgrowth, pointing to autocrine or paracrine mechanisms for its growth-promoting effects. The mRNA encoding amphoterin localizes to axonal processes and we showed recently that its 3'-UTR is sufficient for axonal localization of heterologous transcripts (Donnelly et al., 2013). Here, we show that amphoterin mRNA is transported constitutively into axons of adult DRG neurons. A preconditioning nerve injury increases the levels of amphoterin protein in axons without a corresponding increase in amphoterin mRNA in the axons. A 60 nucleotide region of the amphoterin mRNA 3'-UTR is necessary and sufficient for its localization into axons of cultured sensory neurons. Amphoterin mRNA 3'-UTR is also sufficient for axonal localization in distal axons of DRG neurons in vivo. Overexpression of axonally targeted amphoterin mRNA increases axon outgrowth in cultured sensory neurons, but axon growth is not affected when the overexpressed mRNA is restricted to the cell body.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Células Receptoras Sensoriais / Axônios / Biossíntese de Proteínas / RNA Mensageiro / Regulação da Expressão Gênica / Proteína HMGB1 Limite: Animals Idioma: En Revista: J Neurosci Ano de publicação: 2015 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Células Receptoras Sensoriais / Axônios / Biossíntese de Proteínas / RNA Mensageiro / Regulação da Expressão Gênica / Proteína HMGB1 Limite: Animals Idioma: En Revista: J Neurosci Ano de publicação: 2015 Tipo de documento: Article