[The development of kit for hybridization extraction of DNA and RNA of agents of hemotransmissive infections from serum and blood plasma].
Klin Lab Diagn
; 59(10): 40-5, 2014 Oct.
Article
em Ru
| MEDLINE
| ID: mdl-25884079
ABSTRACT
To decrease dependence of effectiveness of isolation of nucleic acids of composition and amount of applied sample a kit was developed for hybridization extraction of DNA HBV RNA HCV and RNA HIV from blood serum in two formats--using up to 250 mkl and up to 1 ml of sample. This kit, in complex with kits for detection using polymerase chain reaction technique in real-time, forms a test characterized by high analytical sensitivity i.e. HBV50 copies per ml, HCV37.5 copies per ml, HIV 13 copies per ml. The developed kit for extraction of target nucleic acids permits to get rid of total DNA and inhibited effect of heparin. It can be adapted for application wit factors B and automated stations of sample preparation.
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Coleções:
01-internacional
Contexto em Saúde:
2_ODS3
Base de dados:
MEDLINE
Assunto principal:
Kit de Reagentes para Diagnóstico
/
DNA Viral
/
Infecções por HIV
/
Hepatite C
/
Hepatite B
Limite:
Humans
Idioma:
Ru
Revista:
Klin Lab Diagn
Ano de publicação:
2014
Tipo de documento:
Article