Rapid generation of endogenously driven transcriptional reporters in cells through CRISPR/Cas9.

Rojas-Fernandez, Alejandro; Herhaus, Lina; Macartney, Thomas; Lachaud, Christophe; Hay, Ronald T; Sapkota, Gopal P

Rojas-Fernandez, Alejandro; Herhaus, Lina; Macartney, Thomas; Lachaud, Christophe; Hay, Ronald T; Sapkota, Gopal P.

Afiliação

Rojas-Fernandez A; Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dow Street, Dundee, DD1 5EH, Scotland, United Kingdom.
Herhaus L; MRC Protein Phosphorylation and Ubiquitylation Unit, College of Life Sciences, University of Dundee, Dow Street, Dundee, DD1 5EH, Scotland, United Kingdom.
Macartney T; MRC Protein Phosphorylation and Ubiquitylation Unit, College of Life Sciences, University of Dundee, Dow Street, Dundee, DD1 5EH, Scotland, United Kingdom.
Lachaud C; MRC Protein Phosphorylation and Ubiquitylation Unit, College of Life Sciences, University of Dundee, Dow Street, Dundee, DD1 5EH, Scotland, United Kingdom.
Hay RT; Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dow Street, Dundee, DD1 5EH, Scotland, United Kingdom.
Sapkota GP; MRC Protein Phosphorylation and Ubiquitylation Unit, College of Life Sciences, University of Dundee, Dow Street, Dundee, DD1 5EH, Scotland, United Kingdom.

Sci Rep ; 5: 9811, 2015 Apr 29.

Article em En | MEDLINE | ID: mdl-25922883

Assuntos

Sistemas CRISPR-Cas/genética; Repetições Palindrômicas Curtas Agrupadas e Regularmente Espaçadas/genética; Genes Reporter/genética; Transcrição Gênica/genética; Linhagem Celular Tumoral; Fatores de Troca do Nucleotídeo Guanina/genética; Humanos; Inibidor 1 de Ativador de Plasminogênio/genética; Regiões Promotoras Genéticas/genética; Fator de Crescimento Transformador beta/genética; Transgenes/genética; Fatores ras de Troca de Nucleotídeo Guanina

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Transcrição Gênica / Genes Reporter / Repetições Palindrômicas Curtas Agrupadas e Regularmente Espaçadas / Sistemas CRISPR-Cas Limite: Humans Idioma: En Revista: Sci Rep Ano de publicação: 2015 Tipo de documento: Article

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