In vitro differentiation of sperm from male germline stem cell.
Genet Mol Res
; 14(2): 2964-9, 2015 Apr 10.
Article
em En
| MEDLINE
| ID: mdl-25966059
ABSTRACT
In this study, we developed a model of sperm in vitro differentiation to study the mechanism of spermatogenesis. We isolated newborn male germ cells for in vitro differentiation. We found that after 4-5 weeks of culture, sperm-like cells were occasionally observed in cell co-culture and the feed-layer. After 1-2 weeks of culture followed by 4-5 weeks of co-culture, sperm-like cells were observed.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Espermatogênese
/
Espermatozoides
Limite:
Animals
Idioma:
En
Revista:
Genet Mol Res
Ano de publicação:
2015
Tipo de documento:
Article