In vitro differentiation of sperm from male germline stem cell.

Zheng, P; Zhao, X W; Zheng, X M; Khalid, A; Zhao, Q; Zhang, G X

Zheng, P; Zhao, X W; Zheng, X M; Khalid, A; Zhao, Q; Zhang, G X.

Afiliação

Zheng P; College of Animal Science and Technology, Northeast Agricultural University, Harbin, China.
Zhao XW; College of Animal Science and Technology, Northeast Agricultural University, Harbin, China.
Zheng XM; College of Animal Science and Technology, Northeast Agricultural University, Harbin, China.
Khalid A; College of Animal Science and Technology, Northeast Agricultural University, Harbin, China.
Zhao Q; College of Animal Science and Technology, Northeast Agricultural University, Harbin, China.
Zhang GX; College of Animal Science and Technology, Northeast Agricultural University, Harbin, China gxzhang@neau.edu.cn.

Genet Mol Res ; 14(2): 2964-9, 2015 Apr 10.

Article em En | MEDLINE | ID: mdl-25966059

ABSTRACT

ABSTRACT

In this study, we developed a model of sperm in vitro differentiation to study the mechanism of spermatogenesis. We isolated newborn male germ cells for in vitro differentiation. We found that after 4-5 weeks of culture, sperm-like cells were occasionally observed in cell co-culture and the feed-layer. After 1-2 weeks of culture followed by 4-5 weeks of co-culture, sperm-like cells were observed.

Assuntos

Espermatogênese/fisiologia; Espermatozoides/citologia; Animais; Animais Recém-Nascidos; Bovinos; Diferenciação Celular/fisiologia; Técnicas de Cocultura/métodos; Células Germinativas; Masculino; Células de Sertoli/citologia; Espermatogônias/citologia; Células-Tronco/citologia; Técnicas de Cultura de Tecidos/métodos

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Espermatogênese / Espermatozoides Limite: Animals Idioma: En Revista: Genet Mol Res Ano de publicação: 2015 Tipo de documento: Article

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