[Electrochemical methods for biomedical investigations].

ABSTRACT

In the review, authors discussed recently published experimental data concerning highly sensitive electrochemical methods and technologies for biomedical investigations in the postgenomic era. Developments in electrochemical biosensors systems for the analysis of various bio objects are also considered cytochrome P450s, cardiac markers, bacterial cells, the analysis of proteins based on electro oxidized amino acids as a tool for analysis of conformational events. The electroanalysis of catalytic activity of cytochromes P450 allowed developing system for screening of potential substrates, inhibitors or modulators of catalytic functions of this class of hemoproteins. The highly sensitive quartz crystal microbalance (QCM) immunosensor has been developed for analysis of bio affinity interactions of antibodies with troponin I in plasma. The QCM technique allowed real-time monitoring of the kinetic differences in specific interactions and nonspecific sorption, with out multiple labeling procedures and separation steps. The affinity binding process was characterized by the association (ka) and the dissociation (kd) kinetic constants and the equilibrium association (K) constant, calculated using experimental data. Based on the electroactivity of bacterial cells, the electrochemical system for determination of sensitivity of the microbial cells to antibiotics cefepime, ampicillin, amikacin, and erythromycin was proposed. It was shown that the minimally detectable cell number corresponds to 106 CFU per electrode. The electrochemical method allows estimating the degree of E.coli JM109 cells resistance to antibiotics within 2-5 h. Electrosynthesis of polymeric analogs of antibodies for myoglobin (molecularly imprinted polymer, MIP) on the surface of graphite screen-printed electrodes as sensor elements with o- phenylenediamine as the functional monomer was developed. Molecularly imprinted polymers demonstrate selective complementary binding of a template protein molecule (myoglobin) by the "key-lock" principle.