Patient-derived hepatitis C virus inhibits CD4⺠but not CD8⺠T lymphocyte proliferation in primary T cells.
Virol J
; 12: 93, 2015 Jun 19.
Article
em En
| MEDLINE
| ID: mdl-26084511
ABSTRACT
BACKGROUND:
Hepatitis C virus (HCV) can replicate in cells of the immune system and productively propagate in primary T lymphocytes in vitro. We aimed to determine whether exposure to authentic, patient-derived HCV can modify the proliferation capacity, susceptibility to apoptosis and phenotype of T cells.METHODS:
Primary total T cells from a healthy donor were used as targets and plasma-derived HCV from patients with chronic hepatitis C served as inocula. T cell phenotype was determined prior to and at different time points after exposure to HCV. T cell proliferation and apoptosis were measured by flow cytometry-based assays.RESULTS:
The HCV inocula that induced the highest intracellular expression of HCV also caused a greatest shift in the T cell phenotype from predominantly CD4-positive to CD8-positive. This shift was associated with inhibition of CD4+ but not CD8+ T cell proliferation and did not coincide with altered apoptotic death of either cell subset.CONCLUSIONS:
The data obtained imply that exposure to native HCV can have an impact on the relative frequencies of CD4+ and CD8+ T cells by selectively suppressing CD4(+) T lymphocyte proliferation and this may occur in both the presence and the absence of measurable HCV replication in these cells. If the virus exerts a similar effect in vivo, it may contribute to the impairment of virus-specific T cell response by altering cooperation between immune cell subsets.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ativação Linfocitária
/
Linfócitos T CD4-Positivos
/
Hepatite C
/
Hepacivirus
/
Linfócitos T CD8-Positivos
Limite:
Female
/
Humans
/
Male
Idioma:
En
Revista:
Virol J
Ano de publicação:
2015
Tipo de documento:
Article