Double stranded promoter region of BRAF undergoes to structural rearrangement in nearly physiological conditions.
FEBS Lett
; 589(16): 2117-23, 2015 Jul 22.
Article
em En
| MEDLINE
| ID: mdl-26143373
ABSTRACT
The folding of oncogene promoters into non-canonical DNA secondary structures is considered a strategy to control gene expression. Herein, we focused on a 30 bases sequence located upstream of the transcription start site of BRAF (Braf-176) that contains 80% of guanines. We analyzed the structural behavior of the G- and C-rich strands. By the use of spectroscopic and electrophoretic techniques we confirmed that they actually fold into a predominant antiparallel G-quadruplex and into an i-motif, respectively, and that they can coexist at nearly physiological conditions. Finally, the influence of several factors (KCl, pH, PEG200) on the conversion of the double stranded form of the oncogene promoter into the two above mentioned non-canonical structures has been explored.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Modelos Moleculares
/
Regiões Promotoras Genéticas
/
Sequência Rica em GC
/
Proteínas Proto-Oncogênicas B-raf
/
Conformação de Ácido Nucleico
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
2015
Tipo de documento:
Article