Protective effects of SS31 on tBHP induced oxidative damage in 661W cells.
Mol Med Rep
; 12(4): 5026-34, 2015 Oct.
Article
em En
| MEDLINE
| ID: mdl-26165373
ABSTRACT
The present study aimed to investigate the ability of SS31, a novel mitochondriatargeted peptide to protect against tBHPinduced mitochondrial dysfunction and apoptosis in 661W cell lines. The 661W cells were treated with various concentrations of SS31 and an MTT assay was used to determine cell viability. The expression of nitrotyrosine and 8hydroxydeoxyguanosine (8OHdG) was detected using immunofluorescent staining. Apoptosis were assessed using Hoechst staining and an annexin V/propidium iodide flow cytometer. Reactive oxygen species (ROS) were detected using MitoSOXTM with confocal microscopy. Changes in mitochondrial membrane potential were analyzed using flow cytometry. In addition, the release of cytochrome c was analyzed using confocal microscopy. The viability of the cells improved following treatment with SS31 between 100 nM and 1 µM, compared with untreated control group. Compared with the tBHP treatment group (20.0±3.8%), the number of annexin Vpositive cells decreased dosedependently to 13.6±2.6, 9.8±0.5 and 7.4±2.0% in the SS31 treated group at concentrations of 10 nM, 100 nM and 1 µM, respectively. Treatment with SS31 significantly prevented the tBHPinduced expression of nitrotyrosine and 8OHdG, decreased the quantity of mitochondrial ROS, increased mitochondrial potential, and prevented the release of cytochrome c from mitochondria into the cytoplasm. Therefore, the SS31 mitochondriatargeted peptide protected the 661W cells from the sustained oxidative stress induced by tBHP.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oligopeptídeos
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Oxidantes
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Espécies Reativas de Oxigênio
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Células Fotorreceptoras Retinianas Cones
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Terc-Butil Hidroperóxido
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Antioxidantes
Limite:
Animals
Idioma:
En
Revista:
Mol Med Rep
Ano de publicação:
2015
Tipo de documento:
Article