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The Accuracy of p16/Ki-67 and HPV Test in the Detection of CIN2/3 in Women Diagnosed with ASC-US or LSIL.
Possati-Resende, Júlio C; Fregnani, José H T G; Kerr, Ligia M; Mauad, Edmundo C; Longatto-Filho, Adhemar; Scapulatempo-Neto, Cristovam.
Afiliação
  • Possati-Resende JC; Cancer Prevention Department, Barretos Cancer Hospital, Barretos, São Paulo, Brazil.
  • Fregnani JH; Teaching and Research Institute, Barretos Cancer Hospital, Barretos, São Paulo, Brazil.
  • Kerr LM; Pathology Department, Barretos Cancer Hospital, Barretos, São Paulo, Brazil.
  • Mauad EC; Cancer Prevention Department, Barretos Cancer Hospital, Barretos, São Paulo, Brazil.
  • Longatto-Filho A; Molecular Oncology Center, Barretos Cancer Hospital, Barretos, São Paulo, Brazil; Laboratory of Medical Investigation (LIM-14), School of Medicine, University of Sao Paulo, Sao Paulo, Brazil.
  • Scapulatempo-Neto C; Pathology Department, Barretos Cancer Hospital, Barretos, São Paulo, Brazil; Molecular Oncology Center, Barretos Cancer Hospital, Barretos, São Paulo, Brazil.
PLoS One ; 10(7): e0134445, 2015.
Article em En | MEDLINE | ID: mdl-26230097
The objective of this study was to compare the accuracies of double staining for p16/Ki-67 and the molecular test for high-risk HPV (hr-HPV) to identify high-grade cervical intraepithelial neoplasia (CIN2/CIN3) in women with cervical cytology of atypical squamous cells of undetermined significance (ASC-US) and low-grade squamous intraepithelial lesion (LSIL). Data were collected from 201 women who underwent cervical cytology screening in the Barretos Cancer Hospital and their results were categorized as ASC-US (n=96) or LSIL (n=105). All patients underwent colposcopy with or without cervical biopsy for diagnosis of CIN2/CIN3. The hr-HPV test (Cobas 4800 test) and immunocytochemistry were performed to detect biomarkers p16/Ki-67 (CINtec PLUS test). Two samples (1 ASC-US/1 LSIL) were excluded from the analysis due to inconclusive results of the histologic examination. There were 8 cases of CIN2/CIN3 among 95 women with ASC-US (8.4%), and 23 cases of CIN2/CIN3 among 104 women with LSIL (22.1%). In the group of women with ASC-US, the sensitivity and specificity in diagnosing CIN2/CIN3 were 87.5% and 79.5% for the HPV test and 62.5% and 93.1% for p16/Ki-67. Among women with LSIL, the sensitivity and specificity in the diagnosis of CIN2/CIN3 were 87% and 34.7% for the HPV test and 69.6% and 75.3% for immunocytochemistry. Superior performance was observed for p16/Ki-67 double staining, especially among women under 30 for whom the test had an area under the ROC curve of 0.762 (p<0.001). Both p16/Ki-67 double staining and the hr-HPV DNA test had similar performance in predicting high-grade cervical intraepithelial neoplasia among women with ASC-US. The best performance was observed in women aged >30 years. In younger women (≤30 years) with LSIL, p16/Ki-67 had greater accuracy in identifying precursor lesions. Among women >30 years diagnosed with LSIL, the two methods showed similar performance.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Carcinoma de Células Escamosas / Displasia do Colo do Útero / Antígeno Ki-67 / Inibidor p16 de Quinase Dependente de Ciclina / Alphapapillomavirus Tipo de estudo: Diagnostic_studies / Observational_studies / Prevalence_studies / Prognostic_studies / Risk_factors_studies Limite: Female / Humans Idioma: En Revista: PLoS One Ano de publicação: 2015 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Carcinoma de Células Escamosas / Displasia do Colo do Útero / Antígeno Ki-67 / Inibidor p16 de Quinase Dependente de Ciclina / Alphapapillomavirus Tipo de estudo: Diagnostic_studies / Observational_studies / Prevalence_studies / Prognostic_studies / Risk_factors_studies Limite: Female / Humans Idioma: En Revista: PLoS One Ano de publicação: 2015 Tipo de documento: Article