Calcium Carbonate Mineralized Nanoparticles as an Intracellular Transporter of Cytochromeâ
c for Cancer Therapy.
Chem Asian J
; 10(11): 2380-7, 2015 Nov.
Article
em En
| MEDLINE
| ID: mdl-26235642
ABSTRACT
A new intracellular delivery system based on an apoptotic protein-loaded calcium carbonate (CaCO3 ) mineralized nanoparticle (MNP) is described. Apoptosis-inducing cytochromeâ
c (Cytâ
c) loaded CaCO3 MNPs (Cytâ
c MNPs) were prepared by block copolymer mediated in situ CaCO3 mineralization in the presence of Cytâ
c. The resulting Cytâ
c MNPs had a vaterite polymorph of CaCO3 with a mean hydrodynamic diameter of 360.5â
nm and exhibited 60% efficiency for Cytâ
c loading. The Cytâ
c MNPs were stable at physiological pH (pHâ
7.4) and effectively prohibited the release of Cytâ
c, whereas, at intracellular endosomal pH (pHâ
5.0), Cytâ
c release was facilitated. The MNPs enable the endosomal escape of Cytâ
c for effective localization of Cytâ
c in the cytosols of MCF-7 cells. Flow cytometry showed that the Cytâ
c MNPs effectively induced apoptosis of MCF-7 cells. These findings indicate that the CaCO3 MNPs can meet the prerequisites for delivery of cell-impermeable therapeutic proteins for cancer therapy.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Carbonato de Cálcio
/
Citocromos c
/
Nanopartículas
Limite:
Humans
Idioma:
En
Revista:
Chem Asian J
Ano de publicação:
2015
Tipo de documento:
Article