Construction and identification of Complex DNA vaccine of hepatitis B and Toxoplasma gondii.
Int J Clin Exp Med
; 8(6): 9156-61, 2015.
Article
em En
| MEDLINE
| ID: mdl-26309572
ABSTRACT
OBJECTIVE:
To construct and identify multi-gene recombinant expression vector pcDNA3-HBsAg-p30-ROP2.METHOD:
Primers were designed according to the gene sequences of restriction enzyme cutting site of recombinant pcDNA3-p30-ROP2 and hepatitis B surface antigen (HBsAg). The target fragment of HBsAg was amplified and cloned to expression vector pcDNA3-p30-ROP2 by restriction enzyme digestion and ligation. The recombinant expression vector pcDNA3-HBsAg-p30-ROP2 was identified by PCR detection, followed by enzyme restriction and sequencing.RESULTS:
The target fragment of HBsAg was successfully amplified, and the multi-gene eukaryotic expression vector pcDNA3-HBsAg-p30-ROP2 was established. PCR detection and restriction enzyme digestion showed that the length of the target fragment was consistent with the theoretical value. The recombinant expression vector contained the complete sequences of p30-ROP2 compound gene and HBsAg.CONCLUSION:
Multi-gene recombinant expression vector pcDNA3-HBsAg-p30-ROP2 was successfully established. The constructed expression vector could be used to develop multi-gene nucleic acid vaccines.
Texto completo:
1
Coleções:
01-internacional
Contexto em Saúde:
1_ASSA2030
Base de dados:
MEDLINE
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Int J Clin Exp Med
Ano de publicação:
2015
Tipo de documento:
Article